Answer
Yes, that is done inside of the supplicant.conf configuration file.
This example shows how to only set to 2.4GHz channels for ETSI domain.
ctrl_interface=/var/run/wpa_supplicant update_config=1 freq_list=2412 2417 2422 2427 2432 2437 2442 2447 2452 2457 2462 2467 2472 network={ ssid="test" psk=0123456789 proto=RSN key_mgmt=WPA-PSK auth_alg=OPEN }
Of course if the radio also supports the 5Ghz band, 5GHz frequencies (5180, 5200, ...) can also be chosen here.
Note: if this is being done on a 5GHz radio and only 2.4GHz channels are selceted it is not a warranty that the radio will never transmit at 5GHz. Hence, above cannot be used as a method to disable 5GHz on a given radio.