Can I load OTA application code into my BL654 USB Dongle P/N 451-00003?

To be able to load an application code Over the Air into a BL654, you need to enter into Virtual Serial Port Command mode. The only way to enter into this mode is to have access to the VSP pin (SIO_02) and pull it to VCC.

The BL654 USB Dongle P/N 451-00003 doesn't expose VSP pin which is tied to the ground internally. There is hence no way to enter into Virtual Serial Port Command mode and to load an application code Over the Air on that product.